加利福尼亚新生奶牛犊粪便中 A 组和 B 组轮状病毒的流行情况。
Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California.
作者信息
Chinsangaram J, Schore C E, Guterbock W, Weaver L D, Osburn B I
机构信息
Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis, USA.
出版信息
Comp Immunol Microbiol Infect Dis. 1995 Feb;18(2):93-103. doi: 10.1016/0147-9571(95)98850-h.
136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
从加利福尼亚州一个犊牛养殖场和一个奶牛场的47头犊牛采集了136份粪便样本，通过逆转录聚合酶链反应（RT-PCR）检测A组和B组轮状病毒的存在情况。在第1、7和14天从每头犊牛采集样本。在14天内，44头犊牛（94%）A组轮状病毒检测呈阳性，且B组轮状病毒检测呈阳性的犊牛数量出乎意料地高（38头犊牛，81%）。当通过聚丙烯酰胺凝胶电泳（PAGE）检查这些样本时，在21头犊牛中发现了轮状病毒，并且所有这些犊牛都具有A组电泳图谱型。在来自21头犊牛的25份PAGE阳性样本中，17份（68%）为短电泳图谱型，4份（28%）为长电泳图谱型，4份（28%）同时含有短电泳图谱型和长电泳图谱型轮状病毒。在正常犊牛和腹泻犊牛中均发现了B组以及短电泳图谱型和长电泳图谱型A组轮状病毒。
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