组合丙氨酸取代使细胞色素 P450BM3 能够快速优化，用于大底物的选择性羟化。
Combinatorial alanine substitution enables rapid optimization of cytochrome P450BM3 for selective hydroxylation of large substrates.
机构信息
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.
出版信息
Chembiochem. 2010 Dec 10;11(18):2502-5. doi: 10.1002/cbic.201000565.
Abstract
Combinatorial alanine substitution of active site residues in a thermostable cytochrome P450 (BM3) variant was used to generate BM3 variants with activity on large substrates. Selective hydroxylation of methoxymethylated monosaccharides, alkaloids, and steroids was thus made possible. This approach could be generally useful for improving the activity of enzymes that show only limited activity on larger substrates.
摘要
通过组合突变位于热稳定细胞色素 P450（BM3）变体活性部位的丙氨酸残基，生成了对大底物有活性的 BM3 变体。从而实现了对甲氧基甲基化的单糖、生物碱和甾体的选择性羟化。该方法对于提高那些对大底物活性有限的酶的活性可能具有普遍的意义。
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