UMR 145 IRD/UM1: VIH/SIDA et Maladies Associées, UFR des Sciences Pharmaceutiques et Biologiques, Montpellier Cedex, France.
Med Mycol. 2011 Jul;49(5):467-74. doi: 10.3109/13693786.2010.538732. Epub 2010 Nov 26.
We studied the cell wall of a Candida albicans laboratory mutant exhibiting a high minimum inhibitory concentration (MIC; 8 μg ml(-1)) for caspofungin without bearing FKS1 mutations. This strain showed a reduced level of ß 1,3 D glucan (0.43×) and a higher chitin content (2.3×) than a control strain even when grown without caspofungin. No significant over- or under-expression of chitin synthase or chitinase genes was observed. However, point mutations were detected in the chitinase 2 and 3 genes. These mutations, which may affect the enzymatic activity of the encoded protein products involved in the degradation of the chitin, could have led to an increased concentration of that component, allowing the strain to compensate for its low ß 1,3 D glucan content and the effect of caspofungin.
我们研究了一株白色念珠菌实验室突变株的细胞壁，该突变株对卡泊芬净的最低抑菌浓度（MIC；8μg/ml）较高，没有 FKS1 突变。与对照菌株相比，即使在不生长卡泊芬净的情况下，该菌株的β1,3 D 葡聚糖水平降低（0.43×），几丁质含量升高（2.3×）。几丁质合酶或几丁酶基因没有明显的过度或低表达。然而，在几丁质酶 2 和 3 基因中检测到点突变。这些突变可能影响参与几丁质降解的编码蛋白产物的酶活性，从而导致该成分的浓度增加，使该菌株能够补偿其低β1,3 D 葡聚糖含量和卡泊芬净的作用。
