Teutsch H F
Histochemistry. 1978 Aug 25;57(2):107-17. doi: 10.1007/BF00496675.
Methodological studies on the histochemical technique for the demonstration of G6Pase activity showed that the occurrence of common artifacts: morphological destruction, extracellular precipitation of reaction product and nuclear staining are dependent on the concentration of lead nitrate, buffer and substrate. By studying the effects of systematic variation of the incubation media on the histochemical reaction optimal concentrations of either of these components were determined. An improved medium containing 3.6 mM lead nitrate, 40 mM tris-maleate buffer, pH 6.5, 10 mM G6P and 300 mM sucrose was used for the study of G6Pase distribution patterns in liver acini of juvenile and adult rats of both sexes and in those of starved adult female rats. The results obtained indicate sex dependent differences in the functional organization of the liver acinus and furthermore demonstrate the rapid functional adaptability of liver parenchyma to changes of the nutritional situation.
用于显示葡萄糖-6-磷酸酶（G6Pase）活性的组织化学技术的方法学研究表明，常见伪像的出现：形态破坏、反应产物的细胞外沉淀和核染色取决于硝酸铅、缓冲液和底物的浓度。通过研究孵育介质的系统变化对组织化学反应的影响，确定了这些成分中任何一种的最佳浓度。一种改良的介质，含有3.6 mM硝酸铅、40 mM三羟甲基氨基甲烷-马来酸盐缓冲液（pH 6.5）、10 mM G6P和300 mM蔗糖，用于研究雌雄幼年和成年大鼠以及饥饿成年雌性大鼠肝腺泡中G6Pase的分布模式。获得的结果表明肝腺泡功能组织存在性别依赖性差异，并且进一步证明了肝实质对营养状况变化的快速功能适应性。
