Benkoël L, Gulian J M, Bongrand P, Dalmasso C, Xerri L, Brisse J, Sastre B, Chamlian A
Laboratories d'Anatomie Pathologique, Faculté de Médecine, Hopital de Sainte Marguerite, Marseille, France.
J Histochem Cytochem. 1990 Nov;38(11):1565-9. doi: 10.1177/38.11.2170502.
Glucose-6-phosphatase (G6Pase) is a multicomponent system that catalyzes G6P hydrolysis. To determine the specificity of the histochemical reaction of G6Pase, we investigated the inhibitory effect of diethyl pyrocarbonate (DEPC), a specific and very effective inhibitor of the phosphohydrolase component of the G6Pase system, in normal human liver. The inactivation of the histochemical enzymatic activity by DEPC was monitored by determining the mean brightness of the microscopic image and the histogram of light intensity distributions. The results obtained indicate that the histogram is more sensitive than the mean brightness to variations of enzymatic activities, and that the percent of pixels brighter than a convenient level is directly proportional to DEPC concentration. This study indicates that DEPC can be used as an efficient inhibitor of the histochemical reaction of G6Pase.
葡萄糖-6-磷酸酶（G6Pase）是一种催化G6P水解的多组分系统。为了确定G6Pase组织化学反应的特异性，我们研究了焦碳酸二乙酯（DEPC）——一种对G6Pase系统磷酸水解酶成分具有特异性且非常有效的抑制剂——在正常人肝脏中的抑制作用。通过测定显微镜图像的平均亮度和光强度分布直方图来监测DEPC对组织化学酶活性的灭活情况。所得结果表明，对于酶活性的变化，直方图比平均亮度更敏感，并且比某个适宜水平更亮的像素百分比与DEPC浓度成正比。本研究表明，DEPC可作为G6Pase组织化学反应的有效抑制剂。
