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肝腺泡组织化学定义区域中葡萄糖-6-磷酸酶活性的定量测定。

Quantitative determination of G6Pase activity in histochemically defined zones of the liver acinus.

作者信息

Teutsch H F

出版信息

Histochemistry. 1978 Dec 13;58(4):281-8. doi: 10.1007/BF00495384.

DOI:10.1007/BF00495384
PMID:216655
Abstract

Qualitative histochemical G6Pase distribution patterns obtained with an improved method (Teutsch, 1978) served as the basis for a zonal microdissection of the liver acinus. G6Pase activity was determined quantitatively in tissue samples of zones 1 and 3 by a microfluorometric method (Burch et al., 1978). Using a correlation system it could be demonstrated that the histochemical distribution pattern obtained with the improved method was in better agreement with quantitatively estimated zonal differences of G6Pase activity, both in fed and starved female rats, than with the Wachstein and Meisel medium (1956). From a total of 50 tissue samples analyzed the following average G6Pase activities were calculated: in fed animals 15.36 +/- 3.48 U/g dry weight in zone 1, and 9.28 +/- 2.15 U/g dry weight in zone 3; in starved female rats 42.50 +/- 8.20 U/g dry weight in zone 1, and 29.25 +/- 5.68 U/g dry weight in zone 3. The qualitative histochemical as well as quantitative zonal differences of G6Pase activities are taken as further support for the hypothesis of metabolic zonation of liver parenchyma.

摘要

采用改良方法（Teutsch，1978年）获得的定性组织化学G6Pase分布模式，作为肝腺泡区域显微切割的基础。通过微量荧光法（Burch等人，1978年）对1区和3区的组织样本中G6Pase活性进行了定量测定。利用一种关联系统可以证明，在喂食和饥饿的雌性大鼠中，用改良方法获得的组织化学分布模式与G6Pase活性的定量估计区域差异的一致性，比与Wachstein和Meisel培养基（1956年）的一致性更好。在总共分析的50个组织样本中，计算出以下平均G6Pase活性：在喂食动物中，1区为15.36±3.48 U/g干重，3区为9.28±2.15 U/g干重；在饥饿的雌性大鼠中，1区为42.50±8.20 U/g干重，3区为29.25±5.68 U/g干重。G6Pase活性的定性组织化学以及定量区域差异，进一步支持了肝实质代谢分区的假说。

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