糖化酵母酿酒酵母NCYC 625中葡糖淀粉酶活性的遗传分析。

A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625.

作者信息

Patel D, Evans I H, Bevan E A

机构信息

Howard Hughes Medical Institute Research Laboratory, Department of Molecular Genetics & Cell Biology, University of Chicago, Illinois 6037.

出版信息

Curr Genet. 1990 Apr;17(4):281-8. doi: 10.1007/BF00314873.

DOI:10.1007/BF00314873

PMID:2111230

Abstract

The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear suppressor genes (SGL) found in some S. cerevisiae tester strains. EMS-induced glucoamylase-negative mutants often contain STA2-suppressor mutations. Depending on the allelic status of GEP1, a nuclear gene which also appears able to antagonise SGL-mediated suppression, STA2 expression can be blocked in petite mutants.

摘要

野生糖化酵母酿酒酵母NCYC 625已被证明对于葡糖淀粉酶指定基因STA2是纯合的。spoII-1定位已将STA2定位于第二条染色体上。STA2的表达在一些但不是所有能够形成孢子的二倍体中受到抑制，并且也受到一些酿酒酵母测试菌株中发现的不连锁核抑制基因（SGL）的抑制。EMS诱导的葡糖淀粉酶阴性突变体通常含有STA2抑制突变。根据GEP1的等位基因状态（GEP1是一个似乎也能够拮抗SGL介导的抑制作用的核基因），STA2的表达在小菌落突变体中可能会被阻断。