Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
Mol Biol Rep. 2011 Oct;38(7):4287-90. doi: 10.1007/s11033-010-0552-x. Epub 2010 Nov 27.
Hepatitis C virus (HCV) can be classified into six major genotypes. The HCV genotypes variability accounts for its geographical distribution, its responses to treatments and the clinical outcomes. The aim of this study was to determine the distribution of HCV genotypes among volunteer blood donors in Thailand. Samples from 135 anti-HCV positive blood donors were analyzed. HCV RNA and genotyping was carried out using nested polymerase chain reaction (PCR) and genotype-specific primer PCR for a portion of the core region. HCV RNA was detected in 109 samples (80.7%). Genotype analysis demonstrated four different genotypes. The most common was genotype 3a (36.7%), followed by genotype 6 (29.4%), 1a (19.3%), 1b (6.4%) and mixed infection (1.8%). Seven samples were untyped (6.4%) in the present study. In several previous reports, the prevalence found in Thailand was HCV genotypes 3, 1 and 6. The present results show an increasing importance of the genotype 6 in HCV infections. This study has also described for the first time in Thailand mixed infections of HCV genotypes.
丙型肝炎病毒 (HCV) 可分为 6 种主要基因型。HCV 基因型的变异性与其地理分布、对治疗的反应和临床结果有关。本研究旨在确定泰国志愿献血者中 HCV 基因型的分布。分析了 135 名抗 HCV 阳性献血者的样本。使用巢式聚合酶链反应 (PCR) 和核心区域部分的基因型特异性引物 PCR 进行 HCV RNA 和基因分型。在 109 个样本中检测到 HCV RNA(80.7%)。基因型分析显示存在四种不同的基因型。最常见的是基因型 3a(36.7%)，其次是基因型 6(29.4%)、1a(19.3%)、1b(6.4%)和混合感染(1.8%)。本研究中有 7 个样本未定型(6.4%)。在以前的一些报告中，泰国发现的 HCV 基因型主要是 3、1 和 6。本研究结果表明，HCV 基因型 6 的重要性日益增加。本研究还首次在泰国描述了 HCV 基因型的混合感染。
