Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Syst Appl Microbiol. 2010 Dec;33(8):444-50. doi: 10.1016/j.syapm.2010.08.006. Epub 2010 Nov 5.
Gram-negative, facultatively anaerobic bacterial strains were consistently isolated from oak trees displaying symptoms of extensive stem bleeding. In Britain, this disorder is called Acute Oak Decline (AOD). A similar condition has been noted on species of Mediterranean oak in Spain. The identity of bacterial isolates from symptomatic trees in both countries was investigated using molecular techniques and phenotypic assays. 16S rRNA gene sequencing indicated that the strains were most closely related to the genera Serratia, Kluyvera, Klebsiella and Raoultella (all>97%). Phylogenetic analysis revealed that the strains formed a distinct lineage within the family Enterobacteriaceae, which was confirmed by both gyrB- and rpoB-gene sequencing. DNA-DNA hybridization confirmed that the strains belonged to a single taxon which could also be differentiated phenotypically from its closest phylogenetic neighbours. The phylogenetic and phenotypic data both demonstrated that the strains isolated from oak represented a novel genus and species within the family Enterobacteriaceae for which the name Gibbsiella quercinecans gen. nov., sp. nov. (type strain=FRB 97(T)=LMG 25500(T)=NCPPB 4470(T)) is proposed.
从表现出大量茎出血症状的橡树中分离到了革兰氏阴性、兼性厌氧的细菌菌株。在英国，这种疾病被称为急性橡树衰退（AOD）。在西班牙的地中海橡树物种中也注意到了类似的情况。使用分子技术和表型分析研究了来自两国症状树的细菌分离株的身份。16S rRNA 基因测序表明，这些菌株与沙雷氏菌属、克吕沃尔氏菌属、克雷伯氏菌属和劳氏菌属（均>97%）最为密切相关。系统发育分析表明，这些菌株在肠杆菌科内形成了一个独特的谱系，这一点通过gyrB 和 rpoB 基因测序得到了证实。DNA-DNA 杂交证实，这些菌株属于一个单一的分类群，也可以通过表型与最接近的系统发育邻居区分开来。系统发育和表型数据均表明，从橡树中分离到的菌株代表了肠杆菌科中的一个新属和新种，拟名为 Gibbsiella quercinecans gen. nov.，sp. nov.（模式株=FRB 97（T）=LMG 25500（T）=NCPPB 4470（T））。
