Forest Research, Centre for Forestry and Climate Change, Alice Holt Lodge, Farnham, Surrey GU10 4LH, UK.
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2012 Oct;62(Pt 10):2451-2456. doi: 10.1099/ijs.0.037879-0. Epub 2011 Nov 25.
A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus Brenneria, family Enterobacteriaceae, and are most closely related to Brenneria rubrifaciens (97.6 % sequence similarity to the type strain). Multilocus sequence analysis based on four housekeeping genes (gyrB, rpoB, infB and atpD) confirmed their position within the genus Brenneria, while DNA-DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phenotypically from their closest phylogenetic neighbours. The phylogenetic and phenotypic data demonstrate that these isolates from oak with symptoms of AOD represent a novel species in the genus Brenneria, for which the name Brenneria goodwinii sp. nov. (type strain FRB 141(T) = R-43656(T) = BCC 845(T) = LMG 26270(T) = NCPPB 4484(T)) is proposed.
采用多相分类法研究了一组 9 株分离自英国表现出急性栎衰退（AOD）症状的乡土栎树的革兰氏阴性染色、兼性厌氧菌。16S rRNA 基因测序和系统发育分析表明，这些分离株在肠杆菌科布伦纳氏菌属内形成一个独特的谱系，与红棕色布伦纳氏菌（与模式株的序列相似度为 97.6%）最为密切相关。基于 4 个看家基因（gyrB、rpoB、infB 和 atpD）的多位点序列分析证实了它们在布伦纳氏菌属内的位置，而 DNA-DNA 杂交表明这些分离株属于一个单一种群。这些分离株在表型上可与最接近的系统发育近邻区分开来。系统发育和表型数据表明，这些来自具有 AOD 症状的栎树的分离株代表了布伦纳氏菌属中的一个新种，建议将其命名为Brenneria goodwinii sp. nov.（模式株 FRB 141（T）=R-43656（T）=BCC 845（T）=LMG 26270（T）=NCPPB 4484（T））。
