Brady Carrie, Hunter Gavin, Kirk Susan, Arnold Dawn, Denman Sandra
Centre for Research in Bioscience, Faculty of Health and Life Sciences, University of the West of England, Frenchay Campus, Bristol, United Kingdom.
Forest Research, Centre for Ecosystems Society and Biosecurity, Alice Holt Lodge, Surrey, United Kingdom.
Syst Appl Microbiol. 2014 Sep;37(6):396-401. doi: 10.1016/j.syapm.2014.04.005. Epub 2014 May 22.
Gram-negative, facultatively anaerobic bacteria were isolated from symptomatic oak tissue in the UK and USA. Partial gyrB sequencing placed ten strains in the genus Brenneria, with B. goodwinii as the closest phylogenetic relative. The strains were investigated further using a polyphasic approach including MLSA (based on partial gyrB, rpoB, infB and atpD gene sequences), 16S rRNA gene sequencing, DNA-DNA relatedness studies and both phenotypic and chemotaxonomic assays. The MLSA and 16S rRNA gene analyses separated the strains into two groups based on origin, suggesting that they belong to Brenneria as two novel species. However, the DNA-DNA relatedness values revealed a closer relationship between the groups and indicated that they should belong to the same species. As the two groups of strains from the UK and USA can be differentiated from each other phenotypically and by ERIC PCR fingerprints, it is proposed to classify them as novel subspecies of a novel Brenneria species. The name Brenneria roseae sp. nov. (FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)) is proposed, with Brenneria roseae subsp. roseae ssp. nov. (FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)) for the strains from the UK and Brenneria roseae subsp. americana ssp. nov. (FRB 223(T)=LMG 27715(T)=NCPPB 4582(T)) for the strains from the USA.
在英国和美国，从有症状的橡树组织中分离出革兰氏阴性兼性厌氧细菌。部分gyrB基因测序将10个菌株归入布伦纳氏菌属，其中古德温布伦纳氏菌是最接近的系统发育亲缘种。使用多相方法对这些菌株进行了进一步研究，包括多位点序列分析（基于部分gyrB、rpoB、infB和atpD基因序列）、16S rRNA基因测序、DNA-DNA相关性研究以及表型和化学分类分析。多位点序列分析和16S rRNA基因分析根据来源将菌株分为两组，表明它们属于布伦纳氏菌属的两个新物种。然而，DNA-DNA相关性值显示两组之间关系更密切，表明它们应属于同一物种。由于来自英国和美国的两组菌株在表型和ERIC PCR指纹图谱上可以相互区分，因此建议将它们分类为一个新布伦纳氏菌物种的新亚种。提出了玫瑰布伦纳氏菌新种（FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)），其中玫瑰布伦纳氏菌玫瑰亚种新亚种（FRB 222(T)=LMG 27714(T)=NCPPB 4581(T)）用于来自英国的菌株，玫瑰布伦纳氏菌美洲亚种新亚种（FRB 223(T)=LMG 27715(T)=NCPPB 4582(T)）用于来自美国的菌株。
