Brady Carrie, Hunter Gavin, Kirk Susan, Arnold Dawn, Denman Sandra
Centre for Research in Bioscience, Faculty of Health and Life Sciences, University of the West of England, Frenchay Campus, Bristol, United Kingdom.
Forest Research, Centre for Ecosystems Society and Biosecurity, Alice Holt Lodge, Surrey, United Kingdom.
Syst Appl Microbiol. 2014 Sep;37(6):417-22. doi: 10.1016/j.syapm.2014.07.002. Epub 2014 Jul 21.
In 2010, cream-coloured, Gram-negative staining, facultatively anaerobic enterobacteria were isolated from a single black oak tree (Quercus kelloggii) exhibiting decline symptoms in southern California, USA. These 12 isolates were tentatively identified as Gibbsiella quercinecans based on partial gyrB sequencing. Closer examination of the strains using multilocus sequence analysis, based on partial sequences of gyrB, rpoB, infB and atpD genes, and almost complete 16S rRNA gene sequencing suggested that the isolates belong to a novel taxon within the genus Gibbsiella with G. quercinecans as their closest phylogenetic relative. DNA-DNA relatedness studies confirmed that the strains belong to a single taxon in Gibbsiella, which can be differentiated from other members of the genus by several phenotypic traits. Therefore, the name Gibbsiella greigii sp. nov. is proposed for this novel species isolated from symptomatic Q. kelloggii in the USA with FRB 224(T) (=LMG 27716(T)=NCPPB 4583(T)) as the type strain.
2010年，从美国加利福尼亚州南部一棵表现出衰退症状的黑栎树（凯洛格栎）中分离出了米色、革兰氏阴性染色、兼性厌氧的肠杆菌。基于部分gyrB序列，这12株分离菌初步鉴定为栎叶吉氏杆菌。使用多位点序列分析对这些菌株进行更深入的研究，基于gyrB、rpoB、infB和atpD基因的部分序列以及几乎完整的16S rRNA基因测序，结果表明这些分离菌属于吉氏杆菌属内的一个新分类单元，与栎叶吉氏杆菌是最近的系统发育亲缘关系。DNA-DNA相关性研究证实这些菌株属于吉氏杆菌属中的一个单一分类单元，可通过几个表型特征与该属的其他成员区分开来。因此，为从美国有症状的凯洛格栎中分离出的这个新物种提议命名为格氏吉氏杆菌（Gibbsiella greigii sp. nov.），模式菌株为FRB 224(T)（=LMG 27716(T)=NCPPB 4583(T)）。
