Kirsch J A, Springer M S, Krajewski C, Archer M, Aplin K, Dickerman A W
University of Wisconsin Zoological Museum, Madison 53706.
J Mol Evol. 1990 May;30(5):434-48. doi: 10.1007/BF02101115.
A complete suite of comparisons among six bandicoot species and one outgroup marsupial was generated using the hydroxyapatite chromatography method of DNA/DNA hybridization; heterologous comparisons were also made with three other bandicoot taxa. Matrices of delta Tm's, delta modes, and delta T50Hs were generated and corrected for nonreciprocity, homoplasy, and, in the case of delta Tm's, normalized percent hybridization; these matrices were analyzed using the FITCH algorithm in Felsenstein's PHYLIP (version 3.1). Uncorrected and nonreciprocity-corrected matrices were also jackknifed and analyzed with FITCH to test for consistency. Finally, sample scores for delta Tm, delta mode, and delta T50H matrices were bootstrapped and then subjected to phylogenetic analysis. These manipulations were carried out, in part, to address criticisms of the statistics used to summarize DNA/DNA hybridization (especially T50H) and the method itself. However, with the exception of an unresolved trichotomy among the two Echymipera species and Peroryctes longicauda, all trees showed the same branchpoints. Except in the case of the tree generated from reciprocal-corrected delta Tm data, nodes were stable under jackknifing; and, again excepting the above-mentioned trichotomy, all nodes were supported by 95% or more of the bootstrapped trees. These results suggest that, despite arguments to the contrary, all three summary statistics can be valid for DNA/DNA hybridization data. Of taxonomic interest is the placement of Echymipera spp. and Peroryctes longicauda together and separate from the more distant Peroryctes raffrayanus; the genus Peroryctes is thus at least paraphyletic. The trees further grouped Echymipera-plus-Peroryctes as the sister group of Isoodon-plus-Perameles. Limited hybridizations with Macrotis lagotis suggest that its current position as representative of an entirely distinct family of perameloids is correct.
利用DNA/DNA杂交的羟基磷灰石层析法，对六种袋狸物种和一种外群有袋动物进行了一套完整的比较；还与其他三种袋狸分类群进行了异源比较。生成了ΔTm、Δ模式和ΔT50H的矩阵，并对非互反性、同塑性进行了校正，对于ΔTm，还对标准化杂交百分比进行了校正；使用Felsenstein的PHYLIP（版本3.1）中的FITCH算法对这些矩阵进行了分析。未校正和非互反性校正的矩阵也进行了自展检验，并使用FITCH进行分析以检验一致性。最后，对ΔTm、Δ模式和ΔT50H矩阵的样本分数进行了自展检验，然后进行系统发育分析。进行这些操作部分是为了回应人们对用于总结DNA/DNA杂交（特别是T50H）的统计方法及其本身的批评。然而，除了两种刺袋狸属物种和长尾袋狸之间未解决的三分法外，所有树都显示出相同的分支点。除了由互反校正的ΔTm数据生成的树之外，节点在自展检验下是稳定的；并且，再次除了上述三分法外，所有节点都得到了95%或更多自展树的支持。这些结果表明，尽管有相反的观点，但所有这三种总结统计量对于DNA/DNA杂交数据可能都是有效的。在分类学上有趣的是，刺袋狸属物种和长尾袋狸被归在一起，与亲缘关系更远的拉氏袋狸分开；因此，袋狸属至少是并系的。这些树进一步将刺袋狸属加袋狸属作为袋鼬属加袋狸属的姐妹群进行了分组。与大兔耳袋狸的有限杂交表明，它目前作为完全不同的袋狸科代表的位置是正确的。
