Kamp E M, Kimman T G
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
Am J Vet Res. 1988 Nov;49(11):1844-9.
To establish the role of the dermonecrotic toxin (DNT) of Pasteurella multocida in the cause and pathogenesis of atrophic rhinitis, germ-free pigs were inoculated with several strains of P multocida, crude DNT, or purified DNT. In some experiments, the aforementioned inocula were combined with Bordetella bronchiseptica. All DNT-producing P multocida strains induced severe turbinate atrophy. Histologic examination of the remnants of the nasal turbinates revealed intact, but undulated, ciliated epithelium and numerous osteoclasts. Inflammation was minimal or absent. A DNT-producing B bronchiseptica strain induced only mild turbinate atrophy. The lesions were characterized histologically by loss of cilia and ciliated cells and by an infiltration of predominantly mononuclear cells. Bone formation seemed impaired. Turbinate lesions were most severe in pigs infected with a combination of B bronchiseptica and a DNT-producing P multocida strain. Intranasal administration of sterile DNT-containing culture filtrate of P multocida or purified DNT of P multocida did not result in turbinate atrophy. In contrast, turbinate atrophy developed when these preparations were injected IM or when intranasal administration of DNT was preceded by inoculation of B bronchiseptica.
为确定多杀巴斯德菌的皮肤坏死毒素（DNT）在萎缩性鼻炎病因及发病机制中的作用，给无菌猪接种了多株多杀巴斯德菌、粗制DNT或纯化DNT。在一些实验中，将上述接种物与支气管败血波氏杆菌联合使用。所有产DNT的多杀巴斯德菌菌株均引起严重的鼻甲萎缩。鼻甲残余组织的组织学检查显示，纤毛上皮完整但呈波浪状，并有大量破骨细胞。炎症轻微或无炎症。一株产DNT的支气管败血波氏杆菌菌株仅引起轻度鼻甲萎缩。组织学上，病变的特征是纤毛和纤毛细胞丧失，主要为单核细胞浸润。骨形成似乎受损。在感染支气管败血波氏杆菌和产DNT的多杀巴斯德菌菌株组合的猪中，鼻甲病变最为严重。经鼻内给予多杀巴斯德菌含无菌DNT的培养滤液或多杀巴斯德菌纯化DNT不会导致鼻甲萎缩。相反，当这些制剂肌肉注射时，或在经鼻内给予DNT之前先接种支气管败血波氏杆菌时，会出现鼻甲萎缩。
