Abteilung für Systembiochemie, Medizinische Fakultät der Ruhr-Universität Bochum, Bochum, Germany.
Biochem J. 2011 Feb 15;434(1):161-70. doi: 10.1042/BJ20101115.
PIds (phosphoinositides) are phosphorylated derivatives of the membrane phospholipid PtdIns that have emerged as key regulators of many aspects of cellular physiology. We have discovered a PtdIns3P-synthesizing activity in peroxisomes of Saccharomyces cerevisiae and have demonstrated that the lipid kinase Vps34p is already associated with peroxisomes during biogenesis. However, although Vps34 is required, it is not essential for optimal peroxisome biogenesis. The function of Vps34p-containing complex I as well as a subset of PtdIns3P-binding proteins proved to be mandatory for the regulated degradation of peroxisomes. This demonstrates that PtdIns3P-mediated signalling is required for pexophagy.
磷酸肌醇（PIds）是细胞膜磷脂 PtdIns 的磷酸化衍生物，已成为许多细胞生理过程的关键调节因子。我们在酿酒酵母的过氧化物酶体中发现了一种 PtdIns3P 合成酶活性，并证明脂质激酶 Vps34p 在过氧化物酶体的生物发生过程中就已经与之相关联。然而，尽管 Vps34 是必需的，但它对于过氧化物酶体的最佳生物发生并不是必不可少的。含有 Vps34p 的复合物 I 的功能以及一组 PtdIns3P 结合蛋白对于过氧化物酶体的受调控降解是必需的。这表明 PtdIns3P 介导的信号对于pexophagy 是必需的。
