Department of Orthopaedic Surgery, College of Medicine, Dankook University, Cheonan, Korea.
Spine (Phila Pa 1976). 2011 Feb 1;36(3):E155-63. doi: 10.1097/BRS.0b013e3181d8b92a.
In vivo study of a rat spinal cord injury model.
To develop complete transection model of thoracic spinal cord using a polymer sheet and a microtube relevant for delayed transplantation of stem cells.
Stem cell transplantation for the regeneration of spinal cord injuries has used animal models. However, current models suffer from inflammation and leakage, which lessens their usefulness in studying delayed stem cell transplantation.
Thoracic spinal cord at T9 level of adult Sprague-Dawley rats was exposed and a 50:50 sheet of poly(D,L-lactic-coglycolic acid) was inserted, exposed spinal cord was completely transected, and collagen was filled between the gap between the proximal and distal stumps of transected spinal cord. A microtube was placed and fixed between the polymer surfaces facing each other. Behavior testing, magnetic resonance imaging, and myelography were performed to characterize the new complete transection with a gap formation and polymer insertion (GAP) model and to compare the GAP model with the control models. Human mesenchymal stem cells (hMSCs) were transplanted into 3 models and immunohistochemistry and western blot were performed.
The inserted poly(D,L-lactic-coglycolic acid) sheet was completely disappeared 10 weeks after operation, but the inserted microtube remained firmly fixed in its original position. Myelography of the GAP model showed no leakage of contrast medium around the injured spinal cord, whereas magnetic resonance imaging of the severe contusion and simple transection models showed some leakage of contrast medium. Immunohistochemistry and western blot after hMSCs transplantation indicated that transplanted hMSCs survived and migrated well in the GAP model, and the deposition of inflammatory cells in GAP model was less than a simple transection model or severe contusion model.
The developed GAP model is more relevant for delayed transplantation of stem cells for the study of regeneration of spinal cord injury of rats.
大鼠脊髓损伤模型的体内研究。
使用聚合物片和微管开发全横断胸段脊髓模型，用于干细胞的延迟移植。
用于脊髓损伤再生的干细胞移植已经使用了动物模型。然而，目前的模型存在炎症和渗漏问题，这降低了它们在研究延迟干细胞移植中的有用性。
暴露成年 Sprague-Dawley 大鼠 T9 水平的胸段脊髓，插入 50:50 聚（D，L-乳酸-共-乙醇酸）片，暴露的脊髓完全横断，胶原蛋白填充在横断脊髓的近端和远端残端之间的间隙中。放置并固定微管在彼此相对的聚合物表面之间。行为测试、磁共振成像和脊髓造影用于对具有间隙形成和聚合物插入（GAP）模型的新完全横断进行特征描述，并将 GAP 模型与对照模型进行比较。将人骨髓间充质干细胞（hMSCs）移植到 3 种模型中，并进行免疫组织化学和 Western blot 分析。
手术后 10 周，插入的聚（D，L-乳酸-共-乙醇酸）片完全消失，但插入的微管仍牢固地固定在原来的位置。GAP 模型的脊髓造影显示损伤脊髓周围没有造影剂渗漏，而严重挫伤和单纯横断模型的磁共振成像显示出一些造影剂渗漏。hMSCs 移植后的免疫组织化学和 Western blot 分析表明，移植的 hMSCs 在 GAP 模型中存活并良好迁移，GAP 模型中炎症细胞的沉积少于单纯横断模型或严重挫伤模型。
所开发的 GAP 模型更适合于研究大鼠脊髓损伤再生的干细胞延迟移植。
