Instituto Cajal, CSIC, Department of Cellular, Molecular and Developmental Neurobiology, Madrid, Spain.
Glia. 2011 Feb;59(2):278-92. doi: 10.1002/glia.21100.
The olfactory system represents one of the most suitable models to study interactions between the peripheral and central nervous systems. The developing olfactory epithelium (olfactory placode and pit) gives rise to several cell populations that migrate towards the telencephalic vesicle. One of these cell populations, called the Migratory Mass (MM), accompanies the first emerging olfactory axons from the olfactory placode, but the fate of these cells and their contribution to the Olfactory Bulb (OB) populations has not been properly addressed. To asses this issue we performed ultrasound-guided in utero retroviral injections at embryonic day (E) 11 revealing the MM as an early source of Olfactory Ensheathing Cells in later postnatal stages. Employing a wide number of antibodies to identify the nature of the infected cells we described that those cells generated within the MM at E11 belong to different cell populations both in the mesenchyma, where they envelop olfactory axons and express the most common glial markers, and in the olfactory bulb, where they are restricted to the Olfactory Nerve and Glomerular layers. Thus, the data reveal the existence of a novel progenitor class within the MM, potentially derived from the olfactory placode which gives rise to different neural cell population including some CNS neurons, glia and olfactory ensheathing cells.
嗅觉系统是研究外周和中枢神经系统相互作用的最适宜模型之一。发育中的嗅上皮（嗅基板和嗅窝）产生了几种迁移到端脑囊泡的细胞群体。其中一种细胞群体，称为迁移团（MM），伴随第一波从嗅基板中出现的嗅神经轴突，但这些细胞的命运及其对嗅球（OB）细胞群体的贡献尚未得到充分解决。为了解决这个问题，我们在胚胎期 11 天（E）进行了超声引导的子宫内逆转录病毒注射，揭示了 MM 是出生后早期嗅鞘细胞的来源。通过使用大量的抗体来识别感染细胞的性质，我们描述了那些在 E11 日的 MM 中产生的细胞属于不同的细胞群体，既有在嗅鞘细胞包绕嗅神经轴突并表达最常见的神经胶质标志物的间质中，也有在嗅球中，它们仅限于嗅神经和肾小球层。因此，这些数据揭示了 MM 中存在一种新的祖细胞类群，可能来自嗅基板，它产生包括一些中枢神经系统神经元、神经胶质和嗅鞘细胞在内的不同神经细胞群体。
