Dutton R J, Bitton G, Koopman B, Agami O
Department of Environmental Engineering Sciences, University of Florida, Gainesville.
Arch Environ Contam Toxicol. 1990 May-Jun;19(3):395-8. doi: 10.1007/BF01054984.
Except for beta-galactosidase, little is known about the effect of environmental toxicants on enzyme induction. The information could be potentially useful for the development of low-cost and rapid ecotoxicity assays. The effect of toxicants on the de novo biosynthesis of three inducible enzymes, beta-galactosidase and tryptophanase in E. coli and alpha-glucosidase in B. subtilis was investigated. Biosynthesis of alpha-glucosidase was the most sensitive to environmental toxicants, particularly pentachlorophenol and sodium dodecyl sulfate. The sensitivity of B. subtilis to toxicants was further increased when Tween 80 was incorporated in the growth medium.
除β-半乳糖苷酶外，关于环境毒物对酶诱导的影响知之甚少。这些信息可能对开发低成本快速生态毒性检测方法有潜在帮助。研究了毒物对三种可诱导酶从头生物合成的影响，这三种酶分别是大肠杆菌中的β-半乳糖苷酶和色氨酸酶以及枯草芽孢杆菌中的α-葡萄糖苷酶。α-葡萄糖苷酶的生物合成对环境毒物最为敏感，尤其是五氯苯酚和十二烷基硫酸钠。当在生长培养基中加入吐温80时，枯草芽孢杆菌对毒物的敏感性进一步增加。
