Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University Botucatu, Brazil.
Reprod Biol Endocrinol. 2010 Dec 3;8:149. doi: 10.1186/1477-7827-8-149.
The present study aimed to evaluate the efficacy of the hyaluronic acid (HA) binding assay in the selection of motile spermatozoa with normal morphology at high magnification (8400x).
A total of 16592 prepared spermatozoa were selected and classified into two groups: Group I, spermatozoa which presented their head attached to an HA substance (HA-bound sperm), and Group II, those spermatozoa that did not attach to the HA substance (HA-unbound sperm). HA-bound and HA-unbound spermatozoa were evaluated according to the following sperm forms: 1-Normal morphology: normal nucleus (smooth, symmetric and oval configuration, length: 4.75+/-2.8 μm and width: 3.28+/-0.20 μm, no extrusion or invagination and no vacuoles occupied more than 4% of the nuclear area) as well as acrosome, post-acrosomal lamina, neck, tail, besides not presenting a cytoplasmic droplet or cytoplasm around the head; 2-Abnormalities of nuclear form (a-Large/small; b-Wide/narrow; c-Regional disorder); 3-Abnormalities of nuclear chromatin content (a-Vacuoles: occupy >4% to 50% of the nuclear area and b-Large vacuoles: occupy >50% of the nuclear area) using a high magnification (8400x) microscopy system.
No significant differences were obtained with respect to sperm morphological forms and the groups HA-bound and HA-unbound. 1-Normal morphology: HA-bound 2.7% and HA-unbound 2.5% (P = 0.56). 2-Abnormalities of nuclear form: a-Large/small: HA-bound 1.6% vs. HA-unbound 1.6% (P = 0.63); b-Wide/narrow: HA-bound 3.1% vs. HA-unbound 2.7% (P = 0.13); c-Regional disorders: HA-bound 4.7% vs. HA-unbound 4.4% (P = 0.34). 3. Abnormalities of nuclear chromatin content: a-Vacuoles >4% to 50%: HA-bound 72.2% vs. HA-unbound 72.5% (P = 0.74); b-Large vacuoles: HA-bound 15.7% vs. HA-unbound 16.3% (P = 0.36).
The findings suggest that HA binding assay has limited efficacy in selecting motile spermatozoa with normal morphology at high magnification.
本研究旨在评估高倍镜（8400x）下使用透明质酸（HA）结合试验选择具有正常形态的活动精子的效果。
共选择 16592 个准备好的精子并将其分为两组：I 组，精子头部附着在 HA 物质上（HA 结合精子）；II 组，精子头部不附着在 HA 物质上（HA 未结合精子）。HA 结合和 HA 未结合的精子根据以下精子形态进行评估：1-正常形态：正常核（光滑、对称、椭圆形，长度：4.75+/-2.8μm，宽度：3.28+/-0.20μm，无挤压或内陷，无空泡占据核面积的 4%以上）以及顶体、顶体后板、颈部、尾部，同时头部没有细胞质滴或头部周围的细胞质；2-核形态异常（a-大/小；b-宽/窄；c-区域紊乱）；3-核染色质含量异常（a-空泡：占据核面积的>4%至 50%，b-大空泡：占据核面积的>50%）使用高倍镜（8400x）显微镜系统。
HA 结合和 HA 未结合组在精子形态和形态学方面没有显著差异。1-正常形态：HA 结合组为 2.7%，HA 未结合组为 2.5%（P=0.56）。2-核形态异常：a-大/小：HA 结合组 1.6%，HA 未结合组 1.6%（P=0.63）；b-宽/窄：HA 结合组 3.1%，HA 未结合组 2.7%（P=0.13）；c-区域紊乱：HA 结合组 4.7%，HA 未结合组 4.4%（P=0.34）。3.核染色质含量异常：a-空泡>4%至 50%：HA 结合组 72.2%，HA 未结合组 72.5%（P=0.74）；b-大空泡：HA 结合组 15.7%，HA 未结合组 16.3%（P=0.36）。
研究结果表明，HA 结合试验在选择高倍镜下具有正常形态的活动精子方面效果有限。
