Arlian L G, Vyszenski-Moher D L, Merski J A, Ritz H L, Nusair T L, Wilson E R
Department of Biological Sciences, Wright State University, Dayton, Ohio.
Int Arch Allergy Appl Immunol. 1990;91(3):278-84. doi: 10.1159/000235128.
Alcalase and savinase, produced by Bacillus species, are proteolytic enzymes that are used in laundry products and are known to cause respiratory allergy. Antigenic and allergenic characteristics of alcalase and savinase and their potential cross-reactivity were evaluated using crossed immunoelectrophoresis and crossed radioimmunoelectrophoresis. Alcalase exhibited two distinct antigens; one electropositive and one electronegative. The electropositive antigen exhibited some retrograde anodic mobility when coupled with antiserum components. Savinase exhibited one electropositive and two electronegative antigens. The antigens of the two enzymes were clearly different from each other, the three savinase antigens exhibiting greater electrophoretic mobility than the two alcalase antigens. In crossed radioimmunoelectrophoresis studies, only the electropositive antigen of alcalase, its retrograde complex, and the electropositive antigen of savinase bound IgE from the sera of individuals who were skin test positive to one or both enzymes. No evidence of cross-reactivity was observed in heterologous and tandem crossed immunoelectrophoresis studies and heterologous microimmunodiffusion reactions.
芽孢杆菌属产生的碱性蛋白酶和风味蛋白酶是用于洗涤产品的蛋白水解酶，已知会引起呼吸道过敏。使用交叉免疫电泳和交叉放射免疫电泳评估了碱性蛋白酶和风味蛋白酶的抗原性和致敏性特征及其潜在的交叉反应性。碱性蛋白酶表现出两种不同的抗原；一种是电阳性的，一种是电阴性的。当与抗血清成分结合时，电阳性抗原表现出一些逆行阳极迁移率。风味蛋白酶表现出一种电阳性和两种电阴性抗原。两种酶的抗原彼此明显不同，风味蛋白酶的三种抗原比碱性蛋白酶的两种抗原表现出更大的电泳迁移率。在交叉放射免疫电泳研究中，只有碱性蛋白酶的电阳性抗原、其逆行复合物和风味蛋白酶的电阳性抗原与对一种或两种酶皮肤试验呈阳性的个体血清中的IgE结合。在异源和串联交叉免疫电泳研究以及异源微量免疫扩散反应中未观察到交叉反应的证据。
