Faculty of Sciences, University of South Bohemia in České Budějovice, Studentská 13, 370 05 České Budějovice, Czech Republic.
Vet Parasitol. 2011 Mar 10;176(2-3):120-5. doi: 10.1016/j.vetpar.2010.11.010. Epub 2010 Nov 17.
Two species of Cryptosporidium are commonly identified in pigs: Cryptosporidium suis and Cryptosporidium pig genotype II. Detection of Cryptosporidium spp. is routinely based on molecular methods such as polymerase chain reaction (PCR) and subsequent restriction fragment length polymorphism (RFLP) or gene sequencing. However, most of these methods are hampered by low sensitivity to mixed infections. As a solution of this problem, novel species-specific primers were designed and tested in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows), Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology of pig specific Cryptosporidium spp. and its occurrence, which, on the basis of our results, is underestimated.
猪隐孢子虫和猪隐孢子虫 II 型。隐孢子虫属的检测通常基于分子方法，如聚合酶链反应（PCR）和随后的限制性片段长度多态性（RFLP）或基因测序。然而，这些方法大多受到混合感染敏感性低的限制。为了解决这个问题，本研究设计并测试了新型种特异性引物。与属特异性引物相比，我们的引物具有相同的敏感性，但在对来自不同年龄组自然感染猪的 477 个 DNA 样本进行检测时，使用这些种特异性引物检测到更多（1:48）混合感染。我们的结果表明猪对感染的易感性存在年龄依赖性差异。虽然在所有测试的猪（1-12 周龄和母猪）类别中都发现了猪隐孢子虫，但仅在大于 6 周龄的动物中记录到了猪隐孢子虫 II 型。使用种特异性引物可以帮助更好地了解猪特异性隐孢子虫属的流行病学及其发生情况，根据我们的结果，这种情况被低估了。
