Palacio Gustavo, Briese Thomas, Lipkin W Ian
Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
ILAR J. 2010;51(3):245-54. doi: 10.1093/ilar.51.3.245.
Recent advances in nucleic acid diagnostic technologies have revolutionized microbiology by facilitating rapid, sensitive pathogen surveillance and differential diagnosis of infectious diseases. With the expansion and dissemination of genomic sequencing technology scientists are discovering new microbes at an accelerating pace. In this article we review recent progress in the field of pathogen surveillance and discovery with a specific focus on applications in the field of laboratory animal research. We discuss the challenges in proving a causal relationship between the presence of a candidate organism and disease. We also discuss the strengths and limitations of various assay platforms and describe a staged strategy for viral diagnostics. To illustrate the complexity of pursuing pathogen discovery research, we include examples from our own work that are intended to provide insights into the process that led to the selection of particular strategies.
核酸诊断技术的最新进展通过促进对传染病的快速、灵敏病原体监测和鉴别诊断，彻底改变了微生物学。随着基因组测序技术的扩展和传播，科学家们正以越来越快的速度发现新的微生物。在本文中，我们回顾了病原体监测和发现领域的最新进展，特别关注其在实验动物研究领域的应用。我们讨论了证明候选生物体的存在与疾病之间因果关系的挑战。我们还讨论了各种检测平台的优势和局限性，并描述了病毒诊断的分阶段策略。为了说明进行病原体发现研究的复杂性，我们列举了我们自己工作中的例子，旨在深入了解导致选择特定策略的过程。
