Conquet F, Brûlet P
Unité de Génétique Cellulaire du Collège de France et de l'Institut Pasteur, Paris, France.
Mol Cell Biol. 1990 Jul;10(7):3801-5. doi: 10.1128/mcb.10.7.3801-3805.1990.
Murine leukemia inhibitory factor (LIF) protein is a growth factor which has the ability to maintain the developmental potential of pluripotent embryonic stem cells through a specific receptor. We have examined the expression pattern of the LIF gene from the preimplantation stage (3.5 days post coitum) to the midgestation stage (12.5 days post coitum) of the mouse embryo. LIF transcripts were detected at the preimplantation blastocyst stage, whereas no transcripts were detectable in embryonic stem cells. LIF gene transcription continued in the extraembryonic tissue of the 7.5-day and in the placenta of 9.5-, 10.5-, and 12.5-day post coitum embryos. No transcripts were detected in the embryo proper of the corresponding stages. Our results suggest that this growth factor is synthesized in the extraembryonic part of the embryo and acts on the embryonic tissues during early mouse development.
小鼠白血病抑制因子（LIF）蛋白是一种生长因子，它能够通过特定受体维持多能胚胎干细胞的发育潜能。我们研究了小鼠胚胎从植入前阶段（交配后3.5天）到妊娠中期阶段（交配后12.5天）LIF基因的表达模式。在植入前囊胚阶段检测到LIF转录本，而在胚胎干细胞中未检测到转录本。LIF基因转录在交配后7.5天的胚外组织以及交配后9.5天、10.5天和12.5天胚胎的胎盘中持续存在。在相应阶段的胚胎本身未检测到转录本。我们的结果表明，这种生长因子在胚胎的胚外部分合成，并在小鼠早期发育过程中作用于胚胎组织。
