Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, USA.
Nat Struct Mol Biol. 2011 Jan;18(1):91-3. doi: 10.1038/nsmb.1972. Epub 2010 Dec 5.
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
我们已经测试了 200 多种针对果蝇、秀丽隐杆线虫和人类细胞中 57 种不同组蛋白修饰的抗体的特异性和实用性。尽管大多数抗体表现良好，但超过 25%的抗体在斑点印迹或免疫印迹实验中无法通过特异性测试。在特异性抗体中，超过 20%的抗体在染色质免疫沉淀实验中失败。我们建议在使用组蛋白修饰抗体之前进行严格的测试，并提供了一个发布新测试结果的网站（http://compbio.med.harvard.edu/antibodies/）。
