中国养殖场繁育用于出口的食蟹猴（猕猴属）的血统、食蟹猴疟原虫感染率及恒河猴基因混合情况
Ancestry, Plasmodium cynomolgi prevalence and rhesus macaque admixture in cynomolgus macaques (Macaca fascicularis) bred for export in Chinese breeding farms.
作者信息
Zhang Xinjun, Meng Yuhuan, Houghton Paul, Liu Mingyu, Kanthaswamy Sreetharan, Oldt Robert, Ng Jillian, Trask Jessica Satkoski, Huang Ren, Singh Balbir, Du Hongli, Smith David Glenn
机构信息
Department of Anthropology, University of California, Davis, CA, USA.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
出版信息
J Med Primatol. 2017 Apr;46(2):31-41. doi: 10.1111/jmp.12256. Epub 2017 Mar 7.
BACKGROUND
Most cynomolgus macaques (Macaca fascicularis) used in the United States as animal models are imported from Chinese breeding farms without documented ancestry. Cynomolgus macaques with varying rhesus macaque ancestry proportions may exhibit differences, such as susceptibility to malaria, that affect their suitability as a research model.
METHODS
DNA of 400 cynomolgus macaques from 10 Chinese breeding farms was genotyped to characterize their regional origin and rhesus ancestry proportion. A nested PCR assay was used to detect Plasmodium cynomolgi infection in sampled individuals.
RESULTS
All populations exhibited high levels of genetic heterogeneity and low levels of inbreeding and genetic subdivision. Almost all individuals exhibited an Indochinese origin and a rhesus ancestry proportion of 5%-48%. The incidence of P. cynomolgi infection in cynomolgus macaques is strongly associated with proportion of rhesus ancestry.
CONCLUSIONS
The varying amount of rhesus ancestry in cynomolgus macaques underscores the importance of monitoring their genetic similarity in malaria research.
背景
在美国用作动物模型的大多数食蟹猴（猕猴）是从中国养殖场进口的，其谱系无记录。具有不同恒河猴血统比例的食蟹猴可能表现出差异，如对疟疾的易感性，这会影响它们作为研究模型的适用性。
方法
对来自10个中国养殖场的400只食蟹猴的DNA进行基因分型，以确定它们的地区来源和恒河猴血统比例。采用巢式PCR检测法检测抽样个体中的食蟹猴疟原虫感染情况。
结果
所有种群均表现出高度的遗传异质性、低水平的近亲繁殖和遗传分化。几乎所有个体都起源于印度支那，恒河猴血统比例为5% - 48%。食蟹猴中食蟹猴疟原虫感染的发生率与恒河猴血统比例密切相关。
结论
食蟹猴中恒河猴血统数量的差异凸显了在疟疾研究中监测其遗传相似性的重要性。
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