两种 PCR 系统快速检测肾脏组织中钩端螺旋体的比较。
Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue.
机构信息
Federal Institute for Risk Assessment (BfR), Berlin, Germany.
出版信息
Curr Microbiol. 2011 Apr;62(4):1104-6. doi: 10.1007/s00284-010-9829-5. Epub 2010 Dec 4.
PMID:21132297
Abstract
In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive kidney samples.
摘要
在这项研究中，我们比较了两种用于检测钩端螺旋体属的常规 PCR 系统，并评估了当直接应用于小型哺乳动物的肾脏样本时它们的性能。尽管kappa 值为 0.9 表明两种检测方法之间存在高度一致性，但基于外膜脂蛋白基因 lipl32 的 PCR 更为稳健，显示出更多的肾脏样本呈阳性。
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