Department of General Surgery, Shanghai First People's Hospital, College of Medicine, Shanghai Jiao Tong University, 85 Wujin Road, 200080 Shanghai, China.
Med Oncol. 2012 Mar;29(1):77-83. doi: 10.1007/s12032-010-9766-y. Epub 2010 Dec 4.
Expression microarrays are widely used for investigating the candidate molecular targets in human cancer. While genome-wide expression signatures screened by gene set enrichment analysis (GSEA) were not performed in Chinese gastric cancer (GC). To gain new molecular targets for GC, GSEA analysis was performed. In the present study, GSEA were used to pick out differentially expressed gene sets of our database. Total RNA of paired tissue samples (n = 48) and a tissue microarray containing 132 paired tissues were used to further validate expression levels of INHBA and its correction with clinicopathological factors. Upregulated INHBA expression in gastric cancer was screened and further confirmed by qPCR and immunostaining analysis. Increased INHBA expression was significantly correlated with the diameter of cancer and depth of tumor invasion. Patients with higher expression levels of INHBA had a shorter disease-free survival rate. It was effective to gain new molecular targets for GC by GSEA analysis. INHBA may be a poor survival indicator of GC.
表达谱微阵列被广泛用于研究人类癌症的候选分子靶标。然而，在中国胃癌（GC）中没有进行基于基因集富集分析（GSEA）的全基因组表达特征筛选。为了获得 GC 的新分子靶标，进行了 GSEA 分析。在本研究中，GSEA 用于挑选我们数据库中差异表达的基因集。使用配对组织样本的总 RNA（n=48）和包含 132 对组织的组织微阵列进一步验证 INHBA 的表达水平及其与临床病理因素的相关性。通过 qPCR 和免疫组化分析筛选并进一步证实了胃癌中 INHBA 的上调表达。INHBA 的高表达与癌症的直径和肿瘤浸润的深度显著相关。INHBA 表达水平较高的患者无病生存率较低。通过 GSEA 分析可以有效地获得 GC 的新分子靶标。INHBA 可能是 GC 的不良生存指标。
