College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.
Curr Microbiol. 2011 Apr;62(4):1113-22. doi: 10.1007/s00284-010-9835-7. Epub 2010 Dec 7.
Plant growth-promoting bacteria (PGPB) refer to the bacteria beneficial to plants, and they may affect the growth and development of plants directly or indirectly. This article studied the activities of nitrogen fixation and colonization of a strain of PGPB, Klebsiella pneumoniae NG14, which was isolated from the rice root surface. The results showed that NG14 harbouring the nifH gene had nitrogenase activity, (15)N(2)-fixing activity, and was able to colonize on the root surface and within the cavity of root vascular tissues of rice. Using proteomics technology to study the differences and changes of membrane proteins (MP) of NG14 bacterial biofilm in non-biological surface, 28 proteins showing significant differences before and after the formation of bacterial biofilm have been identified, in which the precursors of membrane pore protein OmpC relevant to osmotic stress resistance was up-regulated. This study would have positive significance on further understanding of the direct and indirect promotion effects of PGPB and related mechanisms.
植物促生菌（PGPB）是指对植物有益的细菌，它们可以直接或间接地影响植物的生长和发育。本文研究了从水稻根表面分离得到的一株植物促生菌肺炎克雷伯氏菌 NG14 的固氮和定殖活性。结果表明，携带 nifH 基因的 NG14 具有固氮酶活性、（15）N2-固定活性，并且能够在水稻根表面和根维管束组织内定殖。利用蛋白质组学技术研究 NG14 细菌生物膜在非生物表面上的膜蛋白（MP）差异和变化，鉴定出 28 种在形成生物膜前后表现出显著差异的蛋白质，其中与渗透压应激抗性相关的膜孔蛋白 OmpC 的前体被上调。本研究对进一步了解 PGPB 的直接和间接促进作用及其相关机制具有积极意义。
