Liao N, Bulant M, Nicolas P, Vaudry H, Pelletier G
MRC Group in Molecular Endocrinology, Laval University Medical Center, Quebec, Canada.
Peptides. 1990 Mar-Apr;11(2):397-400. doi: 10.1016/0196-9781(90)90099-q.
Using antisera to two pro-thyrotropin-releasing hormone (pro-TRH)-derived cryptic peptides, we have studies by immunocytochemistry the ultrastructural localization of pro-TRH in the rat raphe nuclei. The same results were obtained with both antisera. Immunostaining was found in cell bodies, dendrites and endings. In cell bodies, the reaction product was restricted to Golgi saccules and dense core vesicles which were very few in number. In dendrites, the staining was rather diffuse without any association with specific organelles. These results suggest that the Golgi apparatus might be involved in pro-TRH processing.
利用针对两种促甲状腺激素释放激素（pro-TRH）衍生的隐蔽肽的抗血清，我们通过免疫细胞化学研究了pro-TRH在大鼠中缝核的超微结构定位。两种抗血清均得到相同结果。在细胞体、树突和终末中均发现免疫染色。在细胞体中，反应产物局限于数量很少的高尔基体囊泡和致密核心囊泡。在树突中，染色较为弥散，与特定细胞器无关联。这些结果表明高尔基体可能参与pro-TRH的加工过程。
