Liao N, Bulant M, Nicholas P, Vaudry H, Pelletier G
MRC Group in Molecular Endocrinology, Laval University Medical Center, CHUL, Quebec, Canada.
Neuropeptides. 1988 Apr;11(3):107-10. doi: 10.1016/0143-4179(88)90078-9.
Using an antiserum to a synthetic peptide corresponding to the sequence Phe-178-Ser-199 of the precursor of thyrotropin-releasing hormone (pro-TRH), we have studied by immunoelectron microscopy the ultrastructural localization of pro-TRH in the rat hypothalamus. In the paraventricular nucleus neuronal cell bodies, dendrites and axons were labelled. The dense core vesicles were strongly immunoreactive. Immunostained cell bodies, dendrites and endings were frequently observed in contact with unidentified neuronal elements. In the median eminence, numerous endings containing positive dense core vesicles were detected. These results suggest that pro-TRH and/or fragment(s) of pro-tRH could play a neuromodulator/neurotransmitter role in the hypothalamus and could also be released into the pituitary portal plexus.
利用针对促甲状腺激素释放激素前体（pro-TRH）中对应于Phe-178-Ser-199序列的合成肽的抗血清，我们通过免疫电子显微镜研究了pro-TRH在大鼠下丘脑中的超微结构定位。在室旁核中，神经元细胞体、树突和轴突都被标记。致密核心囊泡具有强烈的免疫反应性。经常观察到免疫染色的细胞体、树突和末梢与未识别的神经元成分相接触。在正中隆起中，检测到许多含有阳性致密核心囊泡的末梢。这些结果表明，pro-TRH和/或pro-tRH的片段可能在下丘脑中发挥神经调质/神经递质的作用，并且也可能释放到垂体门脉丛中。
