Jackson I M, Wu P, Lechan R M
Science. 1985 Sep 13;229(4718):1097-9. doi: 10.1126/science.3929378.
A rabbit antiserum to a peptide sequence present in the precursor for thyrotropin-releasing hormone (proTRH), deduced from cloned amphibian-skin complementary DNA, was raised by immunization with the synthetic decapeptide Cys-Lys-Arg-Gln-His-Pro-Gly-Lys-Arg-Cys (proTRH-SH). Immunohistochemical studies on rat brain tissue showed staining of neuronal perikarya in the parvicellular division of the paraventricular nucleus of the hypothalamus and the raphe complex of the medulla, identical to that already described for thyrotropin-releasing hormone (TRH). Immunostaining was abolished by preincubation with proTRH-SH (10(-6)M) but not TRH (10(-5)M). Both TRH precursor and TRH were located in neurons of the paraventricular nucleus. However, in contrast to the findings for TRH, no staining was observed in axon terminals of the median eminence. These results suggest that a TRH precursor analogous to that reported in frog skin is present in the rat brain and that TRH in the mammalian central nervous system is a product of ribosomal biosynthesis.
通过用合成十肽Cys-Lys-Arg-Gln-His-Pro-Gly-Lys-Arg-Cys（proTRH-SH）免疫，制备了针对促甲状腺激素释放激素前体（proTRH）中一段肽序列的兔抗血清，该序列由克隆的两栖类皮肤互补DNA推导得出。对大鼠脑组织进行的免疫组织化学研究显示，下丘脑室旁核小细胞部和延髓中缝复合体的神经元胞体有染色，与促甲状腺激素释放激素（TRH）已描述的染色情况相同。用proTRH-SH（10⁻⁶M）预孵育可消除免疫染色，但用TRH（10⁻⁵M）预孵育则不能。TRH前体和TRH都位于室旁核的神经元中。然而，与TRH的研究结果不同，在正中隆起的轴突终末未观察到染色。这些结果表明，大鼠脑中存在与蛙皮肤中报道的类似的TRH前体，并且哺乳动物中枢神经系统中的TRH是核糖体生物合成的产物。
