Perumal P, Selvaraju S, Selvakumar S, Barik A K, Mohanty D N, Das S, Das R K, Mishra P C
Department of Animal Reproduction, Gynaecology and Obstetrics, Orissa University of agriculture and technology, Bhubaneswar, India.
Reprod Domest Anim. 2011 Aug;46(4):636-41. doi: 10.1111/j.1439-0531.2010.01719.x. Epub 2010 Dec 6.
This study was aimed to assess the effect of pre-freeze addition of cysteine hydrochloride and glutathione (GSH) on post-thaw sperm functional parameters and field fertility. The experimental bulls (n = 6) aged 4-6 years were used for this study. A total of 36 ejaculates (six ejaculates per bull) were collected and divided into three groups, group I (control), group II (5 mm cysteine hydrochloride) and group III (5 mm GSH). The extended semen samples with and without additives were filled in mini straw using automatic filling and sealing machine and cryopreserved in liquid nitrogen. Post-freeze seminal traits were recorded after thawing at 37°C for 30 s. The curvilinear velocity (VCL) and amplitude of lateral head displacement values were significantly (p < 0.05) higher in GSH than the cysteine and control groups. Post-thaw plasmalemma integrity was significantly (p < 0.01) higher in GSH group when compared to cysteine and control groups. The loss of acrosomal integrity was significantly (p < 0.01) lower in GSH than in cysteine and control groups. Addition of GSH non-significantly (p = 0.1) improved mitochondrial membrane potential (MMP) (per cent) than control group. This study indicates that the addition of cysteine and GSH suggestive (p = 0.08) of reducing lipid peroxide levels. The conception rate (%) in glutathione group was significantly (p < 0.05) higher than that in cysteine (68 vs 58) and control (49) groups. The post-thaw sperm progressive forward motility (r = 0.4) had moderate, but no significant correlation with conception rate. However, post-thaw straight-line velocity (r = 0.7), loss of acrosomal integrity (r = -0.8) and MMP (r = 0.9) had significant (p < 0.05) correlation with field fertility. This study indicates that the use of glutathione as semen additive may be used for improving post-thaw semen quality and overall augmentation of pregnancy in cows.
本研究旨在评估冻前添加盐酸半胱氨酸和谷胱甘肽（GSH）对解冻后精子功能参数和田间受胎率的影响。本研究使用了6头4至6岁的实验公牛。共采集了36份精液（每头公牛6份精液），并分为三组，第一组（对照组）、第二组（5 mM盐酸半胱氨酸）和第三组（5 mM GSH）。使用自动灌装封口机将添加和未添加添加剂的精液稀释样本装入微型细管中，并在液氮中冷冻保存。在37°C解冻30秒后记录解冻后的精液特征。GSH组的曲线速度（VCL）和头部侧向位移值的幅度显著高于半胱氨酸组和对照组（p < 0.05）。与半胱氨酸组和对照组相比，GSH组解冻后的质膜完整性显著更高（p < 0.01）。GSH组顶体完整性的损失显著低于半胱氨酸组和对照组（p < 0.01）。添加GSH使线粒体膜电位（MMP）（百分比）比对照组有非显著改善（p = 0.1）。本研究表明，添加半胱氨酸和GSH提示（p = 0.08）可降低脂质过氧化物水平。谷胱甘肽组的受胎率（%）显著高于半胱氨酸组（68对58）和对照组（49）（p < 0.05）。解冻后精子的前向渐进运动速度（r = 0.4）与受胎率有中等但不显著的相关性。然而，解冻后的直线速度（r = 0.7）、顶体完整性损失（r = -0.8）和MMP（r = 0.9）与田间受胎率有显著（p < 0.05）相关性。本研究表明，使用谷胱甘肽作为精液添加剂可用于改善解冻后精液质量和提高奶牛的总体妊娠率。
