Perumal P, Vupru Kezhavituo, Rajkhowa C
Animal Reproduction Laboratory, National Research Centre on Mithun (ICAR), Jharnapani, Nagaland 797 106, India.
Vet Med Int. 2013;2013:165348. doi: 10.1155/2013/165348. Epub 2013 Jun 15.
The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliquots and diluted with the TEYC extender. Group 1: semen was without additives (control); groups 2 to 4: semen was diluted with 25 mM, 50 mM, and 100 mM of taurine, respectively. Seminal parameters and enzymatic and biochemical profiles were assessed at 5°C. Inclusion of taurine into diluent resulted in significant (P < 0.05) decreases in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities after liquid storage compared with the control group. Additionally, taurine at 50 mM has significant improvement in quality of mithun semen than taurine at 25 or 100 mM stored in in vitro at 5°C. It was concluded that the possible protective effects of taurine on sperm parameters are from enhancing the function of antioxidant enzymes, preventing efflux of cholesterol from cell membranes and decreased MDA production.
本研究旨在评估牛磺酸对精子活力、存活率、精子总异常率、顶体和质膜完整性、酶学指标（如还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT））以及生化指标（如胆固醇流出和丙二醛（MDA）生成）的影响。每周两次从8头米什米牛采集共50份精液，将精液分成4等份并用TEYC稀释液稀释。第1组：精液无添加剂（对照）；第2至4组：精液分别用25 mM、50 mM和100 mM牛磺酸稀释。在5°C下评估精液参数以及酶学和生化指标。与对照组相比，在稀释液中添加牛磺酸导致液态保存后死精子、异常精子和顶体异常的百分比显著降低（P < 0.05）。此外，在5°C体外保存时，50 mM牛磺酸对米什米牛精液质量的改善显著优于25 mM或100 mM牛磺酸。得出的结论是，牛磺酸对精子参数可能的保护作用来自增强抗氧化酶功能、防止胆固醇从细胞膜流出以及减少MDA生成。
