Unité de Parasitologie Médicale, Centre International de Recherches Médicales de Franceville, B.P. 769, Franceville, Gabon. Lekana
J Ethnopharmacol. 2011 Feb 16;133(3):1103-8. doi: 10.1016/j.jep.2010.11.056. Epub 2010 Dec 4.
As part of a project to identify new compounds active on malarial parasites, we tested the in vitro antiplasmodial activity of nine plants traditionally used to treat malaria symptoms in Haut-Ogooué Province, South-East Gabon.
Dichloromethane and methanolic extracts of each plant were tested for their antiplasmodial activity on two chloroquine-resistant strains of Plasmodium falciparum (FCB and W2), based on lactate dehydrogenase activity. Cytotoxicity was assessed with the MTT test on MRC-5 human diploid embryonic lung cells.
The methanolic extract of Staudtia gabonensis and the dichloromethane extract of Adhatoda latibracteata showed high antiplasmodial activity (IC₅₀<1 μg/ml) and low cytotoxicity, with selectivity indexes of about 58.25 and 16.43, respectively. The methanolic extract of Monodora myristica and the dichloromethane extract of Afromomum giganteum also showed promising activity (1<IC₅₀<10 μg/ml) and low cytotoxicity, with selectivity indexes about 15.70 and 12.48, respectively. Dichloromethane extracts of Monodora myristica and Leonotis Africana showed moderate activity (10<IC₅₀<40 μg/ml), with selectivity indexes about 6.07 and 28.89, respectively. Both extracts of Culcasia lancifolia had IC₅₀ values of 10-40 μg/ml but high cytotoxicity (selectivity indexes <2.77). The methanolic extract of Dorstenia klaineana had moderate antiplasmodial activity (IC₅₀ around 17 μg/ml) but strong cytotoxicity (0.43 μg/ml), giving a selectivity index of about 0.03.
Most extracts of nine selected plants traditionally used to treat malaria in Gabon had interesting antiplasmodial activity in vitro. This supports continued investigations of traditional medicines in the search for new antimalarial agents. The compounds responsible for the observed antiplasmodial effects are under investigation.
作为鉴定抗疟寄生虫新化合物项目的一部分，我们测试了在加蓬东南部上奥果韦省用于治疗疟疾症状的九种传统植物的体外抗疟活性。
基于乳酸脱氢酶活性，用二氯甲烷和甲醇提取物对两种氯喹耐药的恶性疟原虫（FCB 和 W2）进行抗疟活性测试。用 MTT 试验在 MRC-5 人二倍体胚胎肺细胞上评估细胞毒性。
Staudtia gabonensis 的甲醇提取物和 Adhatoda latibracteata 的二氯甲烷提取物表现出高抗疟活性（IC₅₀<1 μg/ml）和低细胞毒性，选择性指数分别约为 58.25 和 16.43。Monodora myristica 的甲醇提取物和 Afromomum giganteum 的二氯甲烷提取物也表现出有希望的活性（1<IC₅₀<10 μg/ml）和低细胞毒性，选择性指数分别约为 15.70 和 12.48。Monodora myristica 的二氯甲烷提取物和 Leonotis Africana 显示出中等活性（10<IC₅₀<40 μg/ml），选择性指数分别约为 6.07 和 28.89。Culcasia lancifolia 的两种提取物的 IC₅₀ 值均为 10-40 μg/ml，但细胞毒性高（选择性指数<2.77）。Dorstenia klaineana 的甲醇提取物具有中等抗疟活性（IC₅₀ 约为 17 μg/ml）但细胞毒性强（0.43 μg/ml），选择性指数约为 0.03。
加蓬传统用于治疗疟疾的九种选定植物的大多数提取物在体外均具有有趣的抗疟活性。这支持继续对传统药物进行研究，以寻找新的抗疟药物。正在研究负责观察到的抗疟作用的化合物。
