Lekana-Douki Jean Bernard, Oyegue Liabagui Sandrine Lydie, Bongui Jean Bernard, Zatra Rafika, Lebibi Jacques, Toure-Ndouo Fousseyni S
Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF) B,P, 769 Franceville, Gabon.
BMC Res Notes. 2011 Nov 23;4:506. doi: 10.1186/1756-0500-4-506.
Malaria remains a major public health problem, especially in tropical and subtropical regions because of the emergence and widespread of antimalarial drug resistance. Traditional medicine represents one potential source of new treatments. Here, we investigated the in vitro antiplasmodial activity of bark extracts from two Fabaceae species (Tetrapleura tertaptera and Copaifera religiosa) traditionally used to treat malaria symptoms in Haut-Ogooué province, Gabon.
The antiplasmodial activity of dichloromethane and methanolic extracts was tested on P. falciparum strains FCB (chloroquine-resistant) and 3D7 (chloroquine-sensitive) and on fresh clinical isolates, using the DELI method. Host cell toxicity was analyzed on MRC-5 human diploid embryonic lung cells using the MTT test.The dichloromethane extracts of the two plants had interesting activity (IC50 between 8.5 ± 4.7 and 13.4 ± 3.6 μg/ml). The methanolic extract of Tetrapleura tetraptera was less active (IC50 around 30 μg/ml) and the methanolic extract of Copaifera religiosa was inactive. The selectivity index (toxicity/antiplasmodial activity) of the dichloromethane extract of Tetrapleura tetraptera was high (around 7), while the dichloromethane extract of Copaifera religiosa had the lowest selectivity (0.6). The mean IC50 values for field isolates were less than 1.5 μg/ml for dichloromethane extracts of both plants, while methanolic extracts of Tetrapleura tetraptera showed interesting activity (IC50 = 13.1 μg/ml). The methanolic extract of Copaifera religiosa was also inactive on field isolates.
Dichloromethane extracts of Tetrapleura tetraptera and Copaifera religiosa, two plants used to treat malaria in Gabon, had interesting antiplasmodial activity in vitro. These data provide a scientific rationale for the traditional use of these plants against malaria symptoms. Bioactivity-guided phytochemical analyses are underway to identify the active compounds.
疟疾仍然是一个主要的公共卫生问题，特别是在热带和亚热带地区，这是由于抗疟药物耐药性的出现和广泛传播。传统医学是新治疗方法的一个潜在来源。在此，我们研究了加蓬上奥果韦省传统上用于治疗疟疾症状的两种豆科植物（四翼四棱豆和宗教苦配巴）树皮提取物的体外抗疟原虫活性。
采用DELI法，对恶性疟原虫菌株FCB（氯喹耐药）和3D7（氯喹敏感）以及新鲜临床分离株测试二氯甲烷和甲醇提取物的抗疟原虫活性。使用MTT试验在MRC-5人二倍体胚胎肺细胞上分析宿主细胞毒性。两种植物的二氯甲烷提取物具有显著活性（IC50在8.5±4.7至13.4±3.6μg/ml之间）。四翼四棱豆的甲醇提取物活性较低（IC50约为30μg/ml），宗教苦配巴的甲醇提取物无活性。四翼四棱豆二氯甲烷提取物的选择性指数（毒性/抗疟原虫活性）较高（约为7），而宗教苦配巴的二氯甲烷提取物选择性最低（0.6）。两种植物二氯甲烷提取物对现场分离株的平均IC50值均小于1.5μg/ml，而四翼四棱豆的甲醇提取物显示出显著活性（IC50 = 13.1μg/ml）。宗教苦配巴的甲醇提取物对现场分离株也无活性。
加蓬用于治疗疟疾的两种植物四翼四棱豆和宗教苦配巴的二氯甲烷提取物在体外具有显著的抗疟原虫活性。这些数据为这些植物传统上用于治疗疟疾症状提供了科学依据。正在进行生物活性导向植物化学分析以鉴定活性化合物。
