Matsubara T, Furukawa S, Yabuta K
Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
Clin Immunol Immunopathol. 1990 Jul;56(1):29-36. doi: 10.1016/0090-1229(90)90166-n.
We investigated 45 patients with Kawasaki disease (KD) and report the first simultaneous determination of tumor necrosis factor (TNF), interleukin 2 receptor (IL-2R) and interferon-gamma (IFN-gamma) in the serum during acute phase. Serum levels of TNF were measured by a sandwich enzyme-linked immunosorbent assay. Serum levels of soluble IL-2R and IFN-gamma were measured by a sandwich enzyme immunoassay and radioimmunoassay, respectively. Serum levels of TNF, IL-2R, and IFN-gamma were seen to increase during the acute phase of KD. In KD patients with coronary-artery lesions (CAL), the percentage of positive cases for TNF (greater than or equal to 10 U/ml), IL-2R (greater than or equal to 1056 U/ml), and IFN-gamma (greater than or equal to 0.3 U/ml) was higher than that in patients without CAL. Our results suggest that aggressive activation of immunocompetent cells develops in KD with CAL.
我们对45例川崎病（KD）患者进行了研究，并首次报告了急性期血清中肿瘤坏死因子（TNF）、白细胞介素2受体（IL-2R）和干扰素-γ（IFN-γ）的同时测定情况。采用夹心酶联免疫吸附测定法检测血清TNF水平。分别采用夹心酶免疫测定法和放射免疫测定法检测血清可溶性IL-2R和IFN-γ水平。在KD急性期，可见血清TNF、IL-2R和IFN-γ水平升高。在患有冠状动脉病变（CAL）的KD患者中，TNF（≥10 U/ml）、IL-2R（≥1056 U/ml）和IFN-γ（≥0.3 U/ml）阳性病例的百分比高于无CAL的患者。我们的结果表明，在伴有CAL的KD中免疫活性细胞发生了强烈激活。
