Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, PR China.
Fish Shellfish Immunol. 2011 Feb;30(2):525-31. doi: 10.1016/j.fsi.2010.11.026. Epub 2010 Dec 4.
Using flow cytometric analysis, the dynamics of surface immunoglobulin positive (sIg+) cells in lymphoid organs of Japanese flounder (Paralichthys olivaceus) reared at 9, 15, 21 and 26 °C, was investigated following intraperitoneal injection with inactivated lymphocystis disease virus (LCDV). The results showed that the percentages of sIg+ cells were suppressed in peripheral blood leucocytes (PBL), spleen leucocytes (SL) and head kidney leucocytes (HKL) from 9 °C to 15 °C immunized groups, and arrived at their peaks (9 °C: 26.12% in PBL, 18.84% in SL, 17.53% in HKL; 15 °C: 38.82% in PBL, 25.38% in SL, 23.95% in HKL) at 9th and 7th week after immunization, respectively. While the proportions of sIg+ cells in PBL, SL and HKL increased most prominent in the 21 °C group and reached the peaks (54.16% in PBL, 30.32% in SL, 30.23% in HKL) at 5th week. The responses of sIg+ cells from 26 °C group were similar to that from 21 °C group and reached the peaks (35.3% in PBL, 26.24% in SL, 21.83% in HKL) at 5th week. Simultaneously, the kinetics of the specific antibody titer against LCDV in sera was determined. It was shown that the antibody response in the 21 °C group was most prominent and reached the peak earliest. These results indicated inactivated LCDV elicited the most powerful immune response when Japanese flounder maintained at the optimal temperature (21 °C) and obtained the most effective immunization, while the response were suppressed at 9 °C, 15 °C or 26 °C.
采用流式细胞术分析方法，研究了在 9、15、21 和 26°C 条件下饲养的牙鲆腹腔注射灭活淋巴囊肿病毒（LCDV）后，其淋巴器官中表面免疫球蛋白阳性（sIg+）细胞的动态变化。结果表明，9°C 至 15°C 免疫组的外周血白细胞（PBL）、脾脏白细胞（SL）和头肾白细胞（HKL）中的 sIg+细胞百分比受到抑制，并在免疫后第 9 周和第 7 周分别达到峰值（9°C：PBL 中为 26.12%，SL 中为 18.84%，HKL 中为 17.53%；15°C：PBL 中为 38.82%，SL 中为 25.38%，HKL 中为 23.95%）。而在 21°C 组中，PBL、SL 和 HKL 中的 sIg+细胞比例增加最为显著，并在第 5 周达到峰值（PBL 中为 54.16%，SL 中为 30.32%，HKL 中为 30.23%）。26°C 组的 sIg+细胞反应与 21°C 组相似，并在第 5 周达到峰值（PBL 中为 35.3%，SL 中为 26.24%，HKL 中为 21.83%）。同时，还测定了血清中针对 LCDV 的特异性抗体滴度的动力学。结果表明，21°C 组的抗体反应最为显著，且最早达到峰值。这些结果表明，当牙鲆保持在最佳温度（21°C）时，灭活的 LCDV 引发了最强烈的免疫反应，并获得了最有效的免疫接种，而在 9°C、15°C 或 26°C 时，反应受到抑制。
