Department of Chemical and Bimolecular Engineering, KAIST, Daejeon, Republic of Korea.
Bioresour Technol. 2011 Feb;102(3):2695-701. doi: 10.1016/j.biortech.2010.10.141. Epub 2010 Nov 17.
The use of volatile fatty acids (VFAs) for microbial lipid accumulation was investigated in flask cultures of Cryptococcus albidus. The optimum culture temperature and pH were 25°C and pH 6.0, respectively, and the highest lipid content (27.8%) was obtained with ammonia chloride as a nitrogen source. The lipid yield coefficient on VFAs was 0.167 g/g of C. albidus with a VFAs (acetic, propionic, butyric acids) ratio of 8:1:1, which was in good agreement with a theoretically predicted lipid yield coefficient of the VFAs as a carbon source. The major fatty acids of the lipids accumulated by C. albidus were similar to those of soybean oil and jatropha oil. A preliminary cost analysis shows that VFAs-based biodiesel production is competitive with current palm and soybean based biodiesels. Further process development for lower aeration cost and higher lipid yield will make this process more economical.
利用挥发性脂肪酸（VFAs）积累微生物油脂，在摇瓶培养中对白腐真菌进行了研究。最佳培养温度和 pH 值分别为 25°C 和 pH 6.0，氯化铵作为氮源可获得最高的油脂含量（27.8%）。VFAs（乙酸、丙酸、丁酸）比例为 8:1:1 时，VFAs 的油脂产率系数为 0.167g/g 白腐真菌，与理论上预测的 VFAs 作为碳源的油脂产率系数相符。白腐真菌积累的油脂的主要脂肪酸与大豆油和麻疯树油相似。初步成本分析表明，基于 VFAs 的生物柴油生产与当前的棕榈油和大豆基生物柴油具有竞争力。进一步降低通气成本和提高油脂产量的工艺开发将使该工艺更具经济性。
