College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Collaborative Innovation Center for Regional Environmental Quality, Tongji University, Shanghai 200092, China.
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Collaborative Innovation Center for Regional Environmental Quality, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2016 Dec;222:75-81. doi: 10.1016/j.biortech.2016.09.100. Epub 2016 Oct 3.
Four mixed volatile fatty acids (VFAs) were used as sole carbon source to culture oleaginous yeast Cryptococcus curvatus by sequencing batch culture strategy. The highest lipid content (42.7%) and concentration (1.77g/L) were achieved when the ratio of VFAs (acetic, propionic, and butyric acids) was 6:3:1. The oleaginous yeast favored to use VFAs for lipid biosynthesis rather than cell proliferation. With regard to the utilization ratio of VFAs, acetic acid reached over 99%, whereas propionic acid was barely 35%. The produced lipids contained nearly 45% of monounsaturated fatty acids, which can be the ideal raw materials for biodiesel production. Additionally, the produced odd-numbered fatty acid content reached 23.6% when the propionate acid content of VFAs was 50%. Further analysis showed that increasing the ratio of acetic acid was most beneficial to cell mass and lipid production, whereas propionic acid and butyric acid were more conducive to lipid and cell mass synthesis, respectively.
四种混合挥发性脂肪酸 (VFAs) 被用作唯一碳源，通过序批培养策略培养产油酵母皱褶假丝酵母。当 VFAs（乙酸、丙酸和丁酸）的比例为 6:3:1 时，达到了最高的脂质含量（42.7%）和浓度（1.77g/L）。产油酵母更倾向于将 VFAs 用于脂质生物合成而不是细胞增殖。就 VFAs 的利用率而言，乙酸的利用率超过 99%，而丙酸的利用率仅为 35%左右。所产生的脂质中含有近 45%的单不饱和脂肪酸，这可以作为生物柴油生产的理想原料。此外，当 VFAs 中的丙酸含量为 50%时，奇数脂肪酸的含量达到 23.6%。进一步分析表明，增加乙酸的比例最有利于细胞质量和脂质的生产，而丙酸和丁酸则分别更有利于脂质和细胞质量的合成。
