College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Collaborative Innovation Center for Regional Environmental Quality, Tongji University, Shanghai 200092, China.
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Collaborative Innovation Center for Regional Environmental Quality, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2017 Oct;241:645-651. doi: 10.1016/j.biortech.2017.05.085. Epub 2017 May 17.
The oleaginous yeast Cryptococcus curvatus ATCC 20509 can use 5-40g/L of acetic, propionic, or butyric acid as sole carbon source to produce lipids. High concentrations (30g/L) of mixed volatile fatty acids (VFAs) were used to cultivate C. curvatus to explore the effects of different ratios of mixed VFAs on lipid production and composition. When mixed VFAs (VFA ratio was 15:5:10) were used as carbon sources, the highest cell mass and lipid concentration were 8.68g/L and 4.93g/L, respectively, which were significantly higher than those when 30g/L of acetic acid was used as sole carbon source. The highest content and yield of odd-numbered fatty acids were 45.1% (VFA ratio was 0:15:15) and 1.62g/L (VFA ratio was 5:15:10), respectively. These results indicate that adjusting the composition ratios of mixed VFAs effectively improves microbial lipid synthesis and the yield of odd-numbered fatty acids.
油脂酵母荚膜红酵母 ATCC 20509 可以利用 5-40g/L 的乙酸、丙酸或丁酸作为唯一碳源来生产油脂。高浓度（30g/L）的混合挥发性脂肪酸（VFA）被用于培养荚膜红酵母，以探讨不同比例的混合 VFA 对产脂和组成的影响。当混合 VFA（VFA 比例为 15:5:10）用作碳源时，细胞质量和油脂浓度分别达到 8.68g/L 和 4.93g/L，显著高于单独使用 30g/L 乙酸作为碳源时的水平。奇数脂肪酸的最高含量和产率分别为 45.1%（VFA 比例为 0:15:15）和 1.62g/L（VFA 比例为 5:15:10）。这些结果表明，调整混合 VFA 的组成比例可有效提高微生物油脂的合成和奇数脂肪酸的产率。
