State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
Bioresour Technol. 2011 Feb;102(3):3360-5. doi: 10.1016/j.biortech.2010.11.065. Epub 2010 Nov 20.
An assessment of seed quality on erythromycin production by recombinant strain Saccharopolyspora erythraea ZL1004 was investigated in 15 l fermenter. Adding 10 g/l corn steep liquor and 30 g/l soybean flour in seed medium were beneficial to improve cell growth, and the maximal biomass reached 36% at 40 h. Enzyme activity in cell showed that the maximal protease and minimum amylase were appeared in this stage. Compared with the control in 50 l fermenter, the cell metabolism with inoculation of the optimized seed cultivation was obviously quicker, and physiological response such as oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were also improved. The maximal erythromycin A production was 9160 U/ml at 215 h, which was increased by 21.63% with respect to the control. It was the first report to integrate cell growth characteristics and physiological response method to assess the seed quality for erythromycin production.
在 15L 发酵罐中评估了重组菌株 Saccharopolyspora erythraea ZL1004 红霉素产量的种子质量。在种子培养基中添加 10g/L 玉米浆和 30g/L 豆粕有利于提高细胞生长，最大生物量在 40h 时达到 36%。细胞中的酶活性表明，在此阶段出现了最大的蛋白酶和最小的淀粉酶。与 50L 发酵罐中的对照相比，优化种子培养接种后的细胞代谢明显加快，摄氧率（OUR）和二氧化碳释放率（CER）等生理反应也得到改善。在 215h 时，红霉素 A 的最大产量为 9160U/ml，与对照相比提高了 21.63%。这是首次将细胞生长特性和生理反应方法集成到评估红霉素生产用种子质量的研究中。
