State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
Bioprocess Biosyst Eng. 2012 Jun;35(5):789-800. doi: 10.1007/s00449-011-0659-z. Epub 2011 Dec 3.
The physiological response of erythromycin fermentation scale-up from 50 L to 132 m(3) scale was investigated. A relatively high oxygen uptake rate (OUR) in early phase of fermentation was beneficial for erythromycin biosynthesis. Correspondingly, the maximal consistency coefficient (K) reflecting non-Newtonian fluid characteristics in 50 L and 132 m(3) fermenter also appeared in same phase. Fluid dynamics in different scale bioreactor was further investigated by real-time computational fluid dynamics modeling. The results of simulation showed that the impeller combination in 50 L fermenter could provide more modest flow field environment compared with that in 132 m(3) fermenter. The decrease of oxygen transfer rate (OTR) in 132 m(3) fermenter was the main cause for impairing cell physiological metabolism and erythromycin biosynthesis. These results were helpful for understanding the relationship between hydrodynamic environment and physiological response of cells in bioreactor during the scale-up of fermentation process.
考察了红霉素发酵从 50L 扩大到 132m³规模的生理反应。发酵早期相对较高的耗氧速率（OUR）有利于红霉素的生物合成。相应地，反映 50L 和 132m³发酵罐中非牛顿流体特性的最大稠度系数（K）也出现在同一阶段。通过实时计算流体动力学建模进一步研究了不同规模生物反应器中的流体动力学。模拟结果表明，与 132m³发酵罐相比，50L 发酵罐中的搅拌器组合可以提供更温和的流场环境。132m³发酵罐中氧传递速率（OTR）的降低是破坏细胞生理代谢和红霉素生物合成的主要原因。这些结果有助于理解发酵过程放大过程中生物反应器中细胞的流体力学生理反应之间的关系。
