Chemistry Department, Faculty of Science, Ain Shams University, Egypt.
Acta Pharm. 2010 Sep;60(3):361-7. doi: 10.2478/v10007-010-0030-y.
In the present study, antioxidant activity of methanolic extracts of the leaves of seven Egyptian Cassia species was investigated using two methods, the phosphomolyb-date method and 1,1 diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity method.· The results revealed that C. glauca is the most potent species and that the activity of other plant species decreases in the following order: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera. Defatted methanolic extract of the most active plant C. glauca was subjected to fractionation using different organic solvents such as CHCl3, EtOAc and BuOH. Antixidant activities of the fractions were investigated and the results showed that ethyl acetate fraction possessed high activity. Total phenolic and flavonoid concentrations of each plant extract were determined using the Folin-Ciocalteu reagent and aluminum chloride. Correlation between radical scavenging capacities of extracts and total phenolic concentration was observed.
在本研究中，使用两种方法研究了七种埃及决明属植物叶的甲醇提取物的抗氧化活性，即磷钼酸盐法和 1,1-二苯基-2-苦基肼自由基（DPPH）清除活性法。结果表明，C. glauca 是最有效的物种，其他植物物种的活性按以下顺序降低：C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera。对最活跃的植物 C. glauca 的脱脂甲醇提取物进行了不同有机溶剂（如 CHCl3、EtOAc 和 BuOH）的分段。研究了各馏分的抗氧化活性，结果表明乙酸乙酯馏分具有较高的活性。使用福林-肖卡尔特试剂和三氯化铝测定了各植物提取物的总酚和类黄酮浓度。观察到提取物的自由基清除能力与总酚浓度之间的相关性。
