Hegazi N M, Hashim A N
Pharmazie. 2016 Sep 1;71(9):544-547. doi: 10.1691/ph.2016.6634.
Chemical investigation of Cassia grandis leaves resulted in the isolation of the new 2-methoxy 6,7,2',6'-tetrahydroxy flavanone 6-O-β-glucoside together with the known flavonol glycosides, kaempferol-3-O-α-rhamnoside, and quercetin 3-O-α-rhamnoside. The structure assign ments were based on conventional analytical methods and confirmed by HRFTESIMS, 1H and 13C NMR, COSY, HSQC and HMBC data. The total phenolic content of the extract was estimated by Folin-Ciocalteu's method. The antioxidant capacity was investigated using DPPH radical scavenging assay. The ethyl acetate and the n-butanol fractions showed poor cytotoxic activity only at high concentrations against the three different cancer cell lines, hepatocellular (HepG-2), breast (MCF-7), and prostate (PC3) by the neutral red uptake assay.
对决明叶进行化学研究，分离得到了新的2-甲氧基-6,7,2',6'-四羟基黄烷酮6-O-β-葡萄糖苷，以及已知的黄酮醇苷、山奈酚-3-O-α-鼠李糖苷和槲皮素3-O-α-鼠李糖苷。结构鉴定基于传统分析方法，并通过高分辨飞行时间电喷雾离子质谱、1H和13C核磁共振、化学位移相关谱、异核单量子相干谱和异核多键相关谱数据得到证实。提取物的总酚含量采用福林-西奥尔特法进行测定。通过二苯基苦味酰基自由基清除试验研究抗氧化能力。乙酸乙酯和正丁醇馏分仅在高浓度时对三种不同癌细胞系，即肝癌细胞（HepG-2）、乳腺癌细胞（MCF-7）和前列腺癌细胞（PC3），通过中性红摄取试验显示出较弱的细胞毒性活性。
