Center for Biomaterial Development, Institute of Polymer Research, GKSS-Forschungszentrum Geesthacht GmbH, Teltow, Germany.
Clin Hemorheol Microcirc. 2010;46(2-3):239-50. doi: 10.3233/CH-2010-1351.
The processing of polymers for blood contacting devices can have a major influence on surface properties. In this study, we fabricated poly(ether imide) (PEI) membranes and films to investigate the effects of the processing on physicochemical surface properties by atomic force microscopy (AFM), scanning electron microscopy, contact angle as well as zeta potential measurements. A static platelet adhesion test was performed to analyze the thrombogenicity of both devices. While contact angle measurements showed similar levels of hydrophobicity and zeta potential values were equivalent, mean surface roughness as well as surface energies in the dispersive part were found to be increased for the PEI membrane. The static platelet adhesion test showed a significantly decreased number of adherent platelets per surface area on the PEI film (178.98 ± 102.70/45000 μm2) compared to the PEI membrane (504 ± 314.27/45000μm2) and, consequently, revealed evidence for higher thrombogenicity of the PEI membrane. This study shows that processing can have a significant effect on platelet adhesion to biomaterials, even though, molar weight was identical. Thrombogenicity of polymer-based cardiovascular devices, therefore, have to be evaluated at the final product level, following the entire processing procedure.
用于血液接触装置的聚合物的处理方法会对表面性能产生重大影响。在这项研究中，我们制备了聚醚酰亚胺（PEI）膜和薄膜，通过原子力显微镜（AFM）、扫描电子显微镜、接触角和zeta 电位测量来研究处理方法对理化表面性能的影响。进行了静态血小板粘附试验，以分析两种装置的血栓形成性。接触角测量显示出相似的疏水性水平，zeta 电位值也相等，但 PEI 膜的平均表面粗糙度和分散部分的表面能都有所增加。静态血小板粘附试验表明，PEI 薄膜上每单位面积的粘附血小板数量明显减少（178.98 ± 102.70/45000 μm2），与 PEI 膜（504 ± 314.27/45000 μm2）相比，因此，PEI 膜的血栓形成性更高。本研究表明，即使摩尔质量相同，处理方法也会对生物材料上的血小板粘附产生重大影响。因此，必须在整个处理过程之后，在最终产品水平上评估基于聚合物的心血管装置的血栓形成性。
