Lanuza M D, Carbajal J A, Villar J, Mir A, Borrás R
Departamento de Microbiología, Facultad de Medicina, Valencia, Spain.
Parasitol Res. 1999 Feb;85(2):93-7. doi: 10.1007/s004360050515.
The protein profile and the antigenic cross-reactivity of 18 axenic isolates of Blastocystis hominis obtained from symptomatic patients with chronic diarrhea (14 isolates) showing no evidence of parasitic etiology and from patients with acute diarrhea attributable in 2 cases to Salmonella spp. were analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of soluble proteins showed the existence of a common profile composed of 31 bands, with molecular weights ranging between 24 and >200 kDa, and minor differences in the proteins of 149, 118, 106, 50, 48, 47, and 30 kDa. These differences allowed us to classify the strains into three related patterns (I-III). In an indirect immunofluorescence assay, all strains were serologically identical, but two related antigenic groups (1 and 2) were found in double-immunodiffusion and Western-blot studies. The isolates of protein patterns I and II belonging to antigenic group 1 were isolated from patients with chronic diarrhea, whereas the four isolates from patients with acute diarrhea were clustered in protein pattern III and in antigenic group 2. These results confirm the protein and antigenic heterogeneity of B. hominis and the existence of demes with different pathogenic roles.
对从患有慢性腹泻的有症状患者（14株分离株）中获得的18株人芽囊原虫无菌分离株的蛋白质谱和抗原交叉反应性进行了分析，这些患者没有寄生虫病因的证据，还分析了2例由沙门氏菌属引起的急性腹泻患者的分离株。可溶性蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示存在一个由31条带组成的共同图谱，分子量在24至>200 kDa之间，并且在149、118、106、50、48、47和30 kDa的蛋白质上存在微小差异。这些差异使我们能够将菌株分为三种相关模式（I-III）。在间接免疫荧光试验中，所有菌株在血清学上是相同的，但在双向免疫扩散和蛋白质印迹研究中发现了两个相关的抗原组（1和2）。属于抗原组1的蛋白质模式I和II的分离株是从慢性腹泻患者中分离出来的，而来自急性腹泻患者的4株分离株聚集在蛋白质模式III和抗原组2中。这些结果证实了人芽囊原虫的蛋白质和抗原异质性以及具有不同致病作用的同类群的存在。
