Chen X Q, Singh M, Ho L C, Tan S W, Yap E H
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
Parasitol Res. 1999 Apr;85(4):343-6. doi: 10.1007/s004360050559.
The protein profiles of Blastocystis hominis, B. lapemi, and B. ratti were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and species could be differentiated by this means as well as by Western-blot analysis with polyclonal antibodies. No intraspecies difference could be distinguished between the two B. hominis isolates or the three B. ratti isolates. Western-blot analysis showed extensive cross-reactivity of B. lapemi and B. hominis antigens with anti-B. ratti serum. Some of the cross-reactive antigens were glycoproteins as determined on the basis of their sensitivity to periodate treatment.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析了人芽囊原虫、拉氏芽囊原虫和鼠芽囊原虫的蛋白质谱，并且可以通过这种方法以及用多克隆抗体进行的蛋白质印迹分析来区分物种。在两个人芽囊原虫分离株或三个鼠芽囊原虫分离株之间未发现种内差异。蛋白质印迹分析表明，拉氏芽囊原虫和人芽囊原虫抗原与人抗鼠芽囊原虫血清有广泛的交叉反应。根据它们对高碘酸盐处理的敏感性确定，一些交叉反应抗原是糖蛋白。
