Institute of Ecological Chemistry, GSF - National Research Center for Environment and Health, Neuherberg, Germany.
Proteomics Clin Appl. 2007 Jul;1(7):650-60. doi: 10.1002/prca.200700195. Epub 2007 Jun 8.
We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE-MS). In the first experiments, the reproducibility of the CE-MS set-up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE-FT-ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain-specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well-known urinary rat peptides, such as collagens, alpha-1-antitrypsin, and serum albumin. The CE-MS-based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non-invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.
我们已经建立并验证了一种使用 CE 与 MS（CE-MS）分析大鼠尿液肽组学的方案。在最初的实验中，评估了 CE-MS 装置和既定制备程序的重现性。为了建立大鼠尿肽组学图谱的第一张图谱，还使用 CE-FT-ICR 对样品进行了分析。随后对来自两个不同品系（WISTAR 和 CD）的独立样本的分析表明存在品系特异性差异，这些差异在盲法评估中得到了验证。MS/MS 显示了来自已知的大鼠尿肽的特定片段的存在，如胶原蛋白、α-1-抗胰蛋白酶和血清白蛋白。基于 CE-MS 的肽组学平台可以为动物模型（如大鼠或小鼠）的体液提供新的见解。与肽鉴定一起，该技术似乎是分析动物模型中药物化合物的毒理学或其他（病理）生理学效应的一种极好的、互补的、非侵入性工具。
