Proteomics Group, Department of Pediatrics, School of Medicine, University of Colorado Health Sciences Center, Aurora, CO, USA.
Proteomics Clin Appl. 2007 Aug;1(8):861-75. doi: 10.1002/prca.200700228. Epub 2007 Jul 13.
Semen is a complex fluid comprising sperm and other products of the testes together with secretions from the accessory sex glands including the prostate, seminal vesicles, and the bulbourethral gland. Studies of the protein components of seminal fluid, with or without the sperm present, can improve our understanding of reproductive biology; have potential clinical applications in the assessment and treatment of infertility; can assist with the diagnosis and management of urological diseases; and, at times, can offer important forensic insights. This review examines the application of proteomic methods to the study of spermatozoa and seminal fluid and highlights some of the unique challenges associated with the collection, fractionation, and analysis of this fluid. The discussion is restricted to issues relating to human semen, although we make occasional reference to important studies from animals.
精液是一种复杂的液体，包含精子和睾丸的其他产物，以及来自附属生殖腺的分泌物，包括前列腺、精囊和尿道球腺。研究精液中的蛋白质成分，无论是否存在精子，都可以增进我们对生殖生物学的理解；在评估和治疗不育症方面具有潜在的临床应用；可以协助诊断和管理泌尿科疾病；并且在某些情况下，可以提供重要的法医学见解。本文综述了蛋白质组学方法在精子和精液研究中的应用，并强调了与这种液体的收集、分离和分析相关的一些独特挑战。讨论仅限于与人类精液有关的问题，尽管我们偶尔会提到来自动物的重要研究。
