Department of Immunotechnology, Lund University, Lund, Sweden.
Proteomics Clin Appl. 2010 Nov;4(10-11):794-807. doi: 10.1002/prca.201000001. Epub 2010 Oct 4.
preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE.
we used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions.
the data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values ≤ 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures.
we have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics.
子痫前期 (PE) 是一种严重的多系统妊娠疾病，其病因尚不清楚，缺乏治疗手段，我们对该病的基本认识仍受到限制。本发现研究的目的是确定候选胎盘组织蛋白生物标志物特征，以进一步解析 PE 的分子特征。
我们使用重组抗体微阵列，针对主要免疫调节水溶性蛋白和膜蛋白，对 25 例子痫前期胎盘组织（n=25）与 11 例正常胎盘（n=11）进行了多重蛋白表达谱分析。此外，我们还对 PE 的三个已知亚组进行了分析，包括早发型子痫前期、晚发型子痫前期以及伴有双侧切迹和宫内生长受限的子痫前期患者。
数据表明，第一代候选 PE 相关胎盘组织蛋白特征已被描绘出来，表明 PE（受试者工作特征曲线 (ROC) AUC 值为 0.83）及其亚组（ROC AUC 值≤0.91）可以区分。值得注意的是，数据表明，所有亚组（伴有双侧切迹和 IUGR 的子痫前期除外）可以进一步分为具有不同炎症特征的新亚组（ROC AUC 值为 1.0）。
我们使用亲和蛋白质组学在分子水平上进一步解析了 PE 的复杂特征。
